5469092038

ENZYMOLOGY OSS 2014

OSS 2014

• Course codę:

• Course title:    Enzymology

• Language of the lecturer:    polish

Course form	Lecture	Classes	Laboratory	Project	Seminar
Number of hours/week^1			1		1
Number of hours/semester^1			15		15
Form of the course completion			attendcmce reports tests		attendcmce prepare of presentation
ECTS credits			2		1
Total Student’s Workload			60		30

•    Level of the course: basie

•    Prereąuisites: working knowledge of biology, microbiology and biochemestry

•    Name, first name and degree of the lecturer/supervisor: Katarzyna Piekarska dr inż.

•    Names, first names and degrees of the team’s members: dr hab. Barbara Kołwzan, dr Waldemar Adamiak

•    Year: I    Semester: I

•    Type of the course: obligatory

•    Aims of the course (effects of the course): Students getting prepared for independent research work on enzyme isolation, purification and stabilization, enzyme kinetics and application in research experiments.

•    Form of the teaching: traditional

•    Course description: Enzymes as biocatalysts. Enzymatic reaction mechanisms. Methods of refining and stabilizing enzymes isolated from natural sources. Enzymatic reaction kinetics. Qualitative and quantitative studies of some enzymes. Contemporary trends in enzyme-based techniąues.

• Lecture:

Particular lectures contents	Number of hours

• Classes - the contents:

1

Seminars - the contents: Enzymes as biocatalysts. Role of energy and Chemical potential in enzyme-based catalysis. Enzymatic reaction mechanisms. Role of enzyme structure in catalytic processes Enzymatic reaction kinetics. Enzyme kinetic models. Enzyme activity regulatory mechanisms. Methods of refining and stabilizing enzymes isolated from natural sources Recombined enzymes obtained in expression systems. Enzyme immobilization. Co-immobilization of enzymes and microorganism cells.