117828251

Essential Oils of Sagę Species 183

Experimental

Plant Materiał

Samples of the two Salina species (S. Imandulifolia and S. triloba) investigated in this study were coilected in the Pharmacognosy Garden of the Medical University (Lublin, Po land) in the vegetation periods of 2007 and 2008. These Salina species are typical of the temperate climatic zonę and are used for diverse purposes. Moreover, both fumish high yields of essential oils, largely composed of terpenes and terpenoids. Because the objective of this study was to enhance the method of first choice for investigation of essential oils from the Salina genus (i.e., HS-GC-MS) by applying ¹³C NMR spec-troscopy, the choice of the essential-oil rich S. lavandulifolia and S. tńloba can be regarded as particularly appropriate. Botany specialists identified both species, and voucher specimens were deposited in the herbarium of the Department of Pharmacognosy, Medical University (Lublin, Poland).

Plant materiał was dried at 35 to 40°C for 40 h in an oven with a forced air flow and the dry materiał obtained was stored in a refrigerator untiJ analysis. Each plant species was weighed and 1-g samples were powdered in a porcelain mortar then placed in 10-mL glass vials stoppered with a sili-cone rubber-Teflon septum. Three replicates of each sample were processed identically by HS-GC-MS.

Headspace Gas Chromatography-Mass Spectrometry

(HS-GC-MS)

Headspace gas chromatography-mass spectrometry (HS-GC-MS) was per-formed with a Tracę 2000 GC and MS Tracę mass detector (ThermoQuest, Waltham, MA, USA) equipped with a CTC Analytics autosampler (Combi PAL, BaseL Switzerland) used in headspace modę. Temperaturę and time of headspace desorption were 70°C and 15 min, respectively. Headspace (0.5 mL) was introduced into a 30 m * 0.25 mm i.d. capillary column coated with a 0.25-pm film of DB-5 (Agilent Technologies, Pało Alto, CA, USA). Helium (p = 100 kPa) was used as carrier gas. The column temperaturę was held at 40°C for 3 min then programmed at 8° min^-1 to 150PC which was held for 15 min. The injector temperaturę was 150°C. The mass spectrometer was fitted with an El source operated at 70 eV. Identification of individual headspace components was based on comparison of their mass spectra with those obtained from standards and those in the National Institute of Stan-dards and Technology (Gaithersburg, MD, USA) software library. Retention times of components of the Salvia samples were also compared with those