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49 Pagc6ofl3 Environ Monit Assess (2017) 189:49

Fig. 3 Growth Vibrioalginolyticus WC PB-11099 (in thc prcscncc of a hcxanc. h hcptanc. c dccanc. and d cyclohcxanc as thc only sourcc of carbon and encigy)

be used for bioremediation and for the treatment of waste oil in the bioreactore.

Generally, E. aurantiacum NB11 3 A strain has sim-ilar growth whatever the alkane tested (Fig. 4), although the strain reaches a higher OD value (1.12) in the presence of decane after 72 h (Fig. 4a). Despite maladjustments with cyclohexane during the first 2 h of growth. the strain has an exponential phase growth where it reaches a plateau phase after 168 h (Fig. 4d).

Halonionas yenusta strain NY-8

According to Rojas et al. (2009), the genus Halonionas is the most abundant bacterial group in marinę emiron-ments. The species most representative of the genus Halom on as is H. venusta (Wang et al. 2009). H. venusta is a halophilic bacterium of the family Halomonadaceae, belongs to the class of Gammaproteobacteria (Franzmann et al. 1988; Dobson and Franzmann 1996). Many studies have re-ported that Halomonas shengHensis (Wang et aL 2007), Halonionas sp. C2SS100, and Pseudomonas sp. C450 R (Mnif et al. 2009; Mnif et al. 2011) use crude oil compounds as substrate for growth.

The genus Halonionas w^ras able to assimilate a w^ride variety of carbon source. Several studies (Okamoto et al. 2(X)4; Mnif et al. 2011) have isolated Halonionas sp. C2SS100 and Pseudomonas. sp. C450R. w'hich degrade from 39 to 96% of the aliphatic fraction C13-C29 of crude oil. H. yenusta NY-8 show^red similar growth ki-netics to other strains, w^rith a very long-latency period of 216 h w^rith hexane (Fig. 5a) and an adaptive difTiculty in the early stage (2 h) with heptane (Fig. 5b) and cyclo-hexane (Fig. 5d). The growth ratę was with slow^r expo-nential phases and Iow OD of 0.38, 0.097, and 0.189, respectively for hexane, heptanes, and cyclohexane (Fig. 5a, b, d). The growth of H. venusta NY-8 in the presence of decane seemed faster with an OD of 0.46 (Fig. 5c) atter 4 days of culture.

Dietzia sp. CNJ898 PL()4

Dietzia spp. was isolated in different em ironments like tropical soils (Von der Weid et al. 2007), alkaline lakes (Duckworth et al. 1998), contaminated land with oil (BorzenkoY et al. 2006), deep marinę sediments (Colquhoun et al. 1998), and the skin and the intestinal tract of marinę fish (Yumoto et al. 2002). Nowadays, many studies have reported that microorganisms as

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