61
ZMIANY WIELKOŚCI MAS CZĄSTECZKOWYCH BIAŁEK PREPARATÓW Z NASION ROŚLIN...
[9] Okada, Y., Okada, M. Scavenging cffect of water soluble protcins in broad beans of free radicals and active oxigen spccics. J. Agric. Food Chem., 46, 1998, 401.
[10] Sanchez-Vioque R., Vioque J., CIcmente A., Pedrochc J., Bautista J., Milian F.: Interaction of chickpea (Cicer arietinum L.) lcgumin with oxidized linoleic acid. J. Agric. Food Chcm. 47, 1999, 813.
[11] Simat T.J., Steinhard H.: Oxidation of frcc tryptophan and tryptophan residues in peptidcs. J. Agric. Food Chem., 46, 1998, 490.
[12] Yamamoto Y., Kato E., Ando A.: Increased antioxidative activity of ovalbuinin by hcat treating in an emulsion of linoleic acid. Biosci. Biotech. Biochem., 60, 1996, 1430.
S u m m a r y
Changcs induced in protein preparations from pea, bean and faba bcan by hydroxyl radicals were de-termincd in this study. Sizc exclusion chromatography showed that the protcins of Icgumes invcstigatcd arc subjected to different changes. In pca and faba been protcins the radicals causcd the inerement of high molecular wcight fraction (330-400 kDa) of rcsulted from fragmentation and polymerisation of Iow mo-lecular weight subunits and, on the contrary, the main fraction of bean proteins (7S) undergoes partial fragmentation. |i§