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B Normoxia Hypoxia
Figurę (Chapitre 3) 1: Hypoxia promotes invadopodia formation
HT-1080 cells were cultured on FiTC-gelatin-coated slides in normoxia or hypoxia for 4 h (B) or 10 h (A,C). (A) Percentage of cells forming invadopodia. (B) Micrographs of actin (green), cortactin (red) nucleus (blue) and merged images are shown. The associated graph shows the number of F-actin-positive and cortactin-positive invadopodia per celi as described under Materials and Methods. (C) Quantification of ECM degradation (area/cell). The associated micrographs show representative ECM degradation area for a single celi. Columns correspond to the mcan ± SEM; ** p < 0.01, *** p < 0.001; scalę bars correspond to 5 pm.
We have previously reported that hypoxia induced an increase in pro-TGFp maturation through up-regulation of the proprotein convertase furin [2]. In addition, TGFP
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