9058009726
niolasses and 0.5 g/L K.2HPO4 was suitable and led to spore concentration of 1.3 x 10 CFU/ml. In 2 L-fermentor, spore concentrations at 48 hr were 1.78 x 104 and 4.03 x 10s CFU/ml for batch and fed-batch process respectively. Addition of molasses during fed-batch cultivation could enhance celi growth on the other liands could impede spore formation. Therefore, in 75-L fed batch experiment, no molasses was fed after the stationary phase was reached, resulting in the spore concentration of 5.73 x 10y CFU/ml at 48 hr.
Keywords: Bacillus subtilis. Feecl Supplement. Fed-batch, Media, Pilot scalę ferment a lion.
corresponding author: Wairuj Dechmahitkul (E-mail: wah nie/ pdt
Introduction: The prohibition of using antibiotic in ani mai farm causes a lot of attention toward live microbial supplements. Unlike antibiotics, they do not have antagonistic or disinfection properties. They help the balance of good microflora in animal intestinal track. There are many reports show that
Methods : BaciUus .subtillis ( TT ST KOO1) was obtained from Thailand Institute of Science and Technology Research. Five formulae of defatted soy bean meal based media was studied in 250 ml shake llask, with 50 ml eiillure volume. at speed of 250 rpm. The
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