4 3 Polymerase Chain Reaction (PCR)

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1

Required large amounts of samples

DNA Fingerprinting

Traces are relatively small

Amplify DNA

Polymerase Chain

Reaction (PCR)

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Thermal cycler

2

Each cycle takes

about 5 minutes

PCR

Typically run 25 to 35

cycles each time

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3

Allows a specific region of DNA

to be repeatedly duplicated

PCR results

25 to 35 cycles

1 million copies

Very small trace

Converted 1 million times

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4

Can analyse very small amounts

PCR results

Saliva from cigarette ends or back of a stamp

Low copy number

9 cells

Barely visible blood stains

36 cells


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