DSC08244 (2)
ektomezenchyma
rostralna
Fig. 3. Comparative rrtorphology of the rostral ectomesen-chyme. (A and B) In both the lamprey and gnetbostomes, the rostral ectomesenchyme is divided into nasal (purple). postoptic (pink)r and mandibular (green) regkms (2j. Postoptic cells surround the premandibular mesoderm (pm). In the lamprey (A), the lower lip {lip) and velum (vel) are deiived from the mandibular crest cells* whereas the upper lip (ułp) is of postoptic origin. In gnathostomes (B). the mandibular arch (ma) differentiates into the maxiUaiy (mx) and mandibular (mand) processes. e. eye; pi, pharyn-geal pouch 1. (C and O) Gene expression pattems are not consistent with the ectomesenchymal morphology. The Dfxl/6 gene (Ught blue) is expressed in the en tire mesen-chyme caudal to the eye in the lamprey (C) but ordy in the mandibular arch in the gnathostome embryo (D). This difference of gene expression corresponds to the distribu-tion of growth factors in the epidermis {blue, FGF8; red. BMP4). (E) FGF8-soaked beads (blue circles) were impiant-ed in the postoptic region of stage 9 chick embryos. (F and G) cDbrf expression after 24 hours of bead implantation (asterisk). The rostral limit of cDtxl expression was shifted rostrally (arrow) in the experimental embryo (G) compared with the control (F). which recetved PBS-soaked beads.
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