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MICROBIOLOGY LAB MANUAŁ ²⁰¹⁶-¹⁷

Experiment No. 1

Safety measures in Microbiology laboratory Rules & Regulations

•    Safety in a microbiology laboratory is important in the prevention of infection that might be caused by the microorganisms being studied. In addition, many of the reagents, equipment, and procedures used are potentially hazardous. Attention to proper procedures and prudent laboratory practices are required for your safety and protection.

•    This course does not require the use of any organisms known to be highly virulent human pathogens. However, some of the organisms used may be potentially pathogenic. This means that, although they may not cause disease in a normal healthy human, they might if the body's antimicrobial defense mechanisms are impaired. In addition, many of the organisms used in this course will be uncharacterized strains isolated from the environment - these cultures should be handled with care, because their virulence is unknown.

•    In addition to organisms, there are some Chemicals used in this laboratory which are potentially harmful. Finally, many procedures involve equipment, glassware, open flames, and sharp objects which can cause injury if used improperly.

•    Although nonę of the organisms, procedures, or materials used in this laboratory are very dangerous, proper safety techniques and precautions should be understood and become part of your reflexive laboratory technique. The following laboratory rules and regulations should be adhered to at all times, NO EXCEPTIONS.

1)    Read and understand each laboratory exercise before you come to class.

2)    Follow precautionary statements given in each exercise.

3)    You should only have the necessary work materiał on the laboratory bench. Coats, backpacks, and other personal belongings will not be allowed on the laboratory bench top. Storę them in a place designated by your instructor or in your laboratory cabinet. This is to prevent cluttering of the workspace and to avoid exposing them to permanent stains, caustic Chemicals, and microorganisms used in the exercises.

4)    Do not eat, drink, smoke, or chew pens in the laboratory.

5)    You must wear shoes while in the laboratory.

6)    Long hair should be tied back so that it does not catch fire in a Bunsen burner flame and does not fali into sterile media.

7)    Wipe your bench top with disinfectant at the beginning and end of every laboratory period.

8)    Wash your hands thoroughly before leaving the laboratory.

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By, Sardar Hussain, Assistant Professor &Head, Dept. Of Biotechnology, CSC, CTA.