Separation condiłions
Columns: Prepbar® 250-50,
LiCnrosorb®
RP-18,10/im Hibar®RT 250-4 LiChrosorb®
RP-18,10 Mm Eluent: Step gradient
0—31.5 minutes melhanol/water (40/50 -v/v)
31.5—42 minutes melhanol/water (95/5 -v/v) Flow(preparative): 80ml/min (analytical): 1 ml/min
Deleclion: UV,254nm
Injecled somple: 3 gin 50 ml
ofmethanol/water (50/50 -v/v)