Inhibicja kompetycyjna
S + E
ES
E + P
I
+
EI
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S], /mM
-1/Km
1/Vmax
Km/Vmax
S
E
CI
0
1
2
3
4
5
0
10
20
30
40
50
v,
µ
m
o
l/
m
in
[S], mM
No I
+ C I
Km
0.5Vmax
K
m
app
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S], /mM
+ C I
No I
-1/Km
1/Vmax
Km/Vmax
-1/K
m
app
K
m
app
/V
max
v
V
max
[S]
K
m
[S]
0
v
V
max
[S]
K
m
[S]
0
1
v
K
m
V
max
1
[S]
1
V
max
0
1
v
K
m
V
max
1
[S]
1
V
max
0
CI – inhibitor kompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
0
0
Inhibicja niekompetycyjna
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S], /mM
-1/Km
1/Vmax
Km/Vmax
0
1
2
3
4
5
0
10
20
30
40
50
v,
µ
m
o
l/
m
in
[S], mM
0.5Vmax
Km
0
1
2
3
4
5
0
10
20
30
40
50
v,
µ
m
o
l/
m
in
[S], mM
No I
+ NCI
Km
0.5Vmax
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S], /mM
+ NC I
No I
-1/Km
1/Vmax
Km/Vmax
K
m
/V
max
app
1/V
max
app
0.5V
max
v
V
max
[S]
K
m
[S]
0
1
v
K
m
V
max
1
[S]
1
V
max
0
v
V
max
[S]
K
m
'[S]
0
1
v
K
m
V
max
1
[S]
'
V
max
0
NCI
S
E
I
I
+
S + E
ES
E + P
EI
ESI
+
S
NCI – inhibitor niekompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
0
0
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S], /mM
-1/Km
1/Vmax
Km/Vmax
Inhibicja akompetycyjna
S E UCI
S + E
ES
E + P
EI
I
+
v
V
max
[S]
K
m
[S]
1
v
K
m
V
max
1
[S]
1
V
max
v
V
max
[S]
K
m
'[S]
1
v
K
m
V
max
1
[S]
'
V
max
0
1
2
3
4
5
0
10
20
30
40
50
v,
µ
m
o
l/
m
in
[S], mM
0.5Vmax
Km
0
1
2
3
4
5
0
10
20
30
40
50
v,
µ
m
o
l/
m
in
[S]. mM
No I
+ UC I
Km
0.5Vmax
K
m
app
0.5V
max
0
0.5
1
1.5
2
2.5
-0.6-0.4-0.2 0 0.2 0.4 0.6 0.8 1
1
/v
,
/µ
m
o
l/
m
in
1/[S]. /mM
+ UC I
No I
-1/Km
1/Vmax
Km/Vmax
1/V
max
app
-1/K
m
app
K
m
app
/V
max
app
UCI – inhibitor akompetycyjny; S – substrat; E – enzym; No I – brak inhibitora
0
0
0
0
0
0