Załącznik Nr 4
do SIWZ
SKŁAD PODŁOŻY
Lp. |
Nazwa podłoża |
Skład |
|
1. |
Zbuforowana woda peptonowa |
Enzmatyczny hydrolizat kazeiny .......................10,0 Chlorek sodu ........................................................5,0 Ortofosforan dwusodowy Na2HPO4 12H20 .......9,0 Ortofosforan jednopotasowy KH2PO4 ................1,5 Woda destylowana ...........................................1000ml |
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2. |
Dodatek selektywny do agaru Palcam na 500 ml pożywki |
Siarczan polimyksyny B.....................................5,0 mg Acriflavine hydrochloride .................................2,5 mg Ceftazidime …………………………………..10,0 mg |
|
3. |
Bulion Frasera |
Proteose pepton ………………..…………………5,0 Trypton …………………………..……………….5,0 ekstrakt mięsny …………………..……………….5,0 Ekstrakt drożdżowy ................................................5,0 Chlorek sodu .........................................................20,0 Fosforan potasu dwuzasadowy ..............................1,35 Disodium hydrogen phosphate .............................12,0 Aesculin ..................................................................1,0 Chlorek litu …………………………………….....3,0 |
|
4. |
Dodatek do Bulionu Frasera na 500 ml |
Kwas nalidiksowy ...........................................10,0 mg Akryflawina ....................................................12,5 mg Cytrynian amonowo-żelazowy .....................250,0 mg
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|
5. |
Dodatek do Bulionu ½ Frasera na 225 ml |
Kwas nalidiksowy ...........................................2,25 mg Akryflawina .................................................2,8125 mg Cytrynian amonowo-żelazowy .....................112,5 mg |
|
6. |
Agar Palcam |
Agar Columbia Base .......................................... 39,0 Ekstrakt drożdżowy.............................................. 3,0 Glukoza ................................................................ 0,5 Aeskulina ............................................................. 0,8 Cytrynian żelazowo-amonowy (III) .................... 0,5 Mannitol ............................................................ 10,0 Czerwień fenolowa .............................................. 0,08 Chlorek litu ........................................................ 15,0 |
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7. |
Podłoże agarowe z ksylozyną i lizyną XLD |
Ekstrakt drożdżowy ............................................. 3,0 L-lysina HCL ....................................................... 5,0 Ksyloza ................................................................ 3,75 Laktoza ................................................................ 7,5 Sacharoza ............................................................... 7,5 Chlorek sodu .......................................................... 5,0 Czerwień fenolowa ................................................0,08 Tiosiarczan sodu .................................................... 6,8 Cytrynian amonowo-żelazowy .............................. 0,8 Agar ............................................................ 12,5-13,5 Dezoksycholan sodu .............................................. 1,0 |
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8. |
Podłoże agarowe z czerwienią i zielenią (modified) BGA |
Ekstrakt mięsny …………………..……………. 5,0 Pepton ................................................................. 10,0 Ekstrakt drożdżowy ............................................. 3,0 Na2HPO4 .............................................................. 1,0 NaH2PO4 ............................................................. 0,6 Agar ................................................................... 15,0 Laktoza ............................................................. 10,0 Sacharoza ........................................................... 10,0 Czerwień fenolowa ............................................. 0,09 Zieleń brylantowa .............................................. 0,005 |
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9. |
Agar odżywczy |
Ekstrakt mięsny …………………..……………. 3,0 Pepton...... ............................................................. 5,0 Chlorek sodu ......................................................... 5,0 Agar ...................................................................... 15,0 |
|
10. |
Podłoże Edwardsa |
Ekstrakt mięsny …………………..………..…. 10,0 Pepton ................................................................. 10,0 Aeskulina .............................................................. 1,0 Chlorek sodu ......................................................... 5,0 Fiolet krystaliczny ........................................... 0,0013 Siarczan talu ......................................................... 0,33 Agar .....................................................................15,0 |
|
11. |
Columbia Agar Base |
Pepton ................................................................. 23,0 Skrobia .................................................................. 1,0 Chlorek sodu ......................................................... 5,0 Agar .....................................................................10,0 |
|
12. |
Podłoże wg Giolitti Cantoni |
Hydrolizat kazeiny ............................................... 10,0 Ekstrakt mięsny …………………..………..….... 5,0 Ekstrakt drożdżowy................................................ 5,0 Chlorek litu ............................................................ 5,0 Chlorek sodu........................................................... 5,0 Mannitol ............................................................... 20,0 Glicyna .................................................................. 1,2 Pirogronian sodu .................................................... 3,0 Tween .................................................................... 1,0 |
|
13. |
Podłoże Sabourada z chloramfenikolem |
Ekstrakt drożdżowy............................................... 2,0 Aminobak ............................................................. 3,0 Pepton SP .............................................................. 3,0 Pepton K ............................................................... 3,0 Glukoza ................................................................ 19,0 Potasu fosforan II zasadowy .................................. 0,5 Potasu fosforan I zasadowy ................................... 0,5 Chloramfenikol ...................................................... 0,5 Agar ..................................................................... 13,0 |
|
14. |
Podłożywka Willis Hobbs (podstawowa) |
Laktoza ................................................................ 8,6 Pepton ..................................................................10,0 Wyciąg mięsny ...................................................... 3,0 NaCI ...................................................................... 5,0 Czerwień obojętna ......................................... 0,0263 Agar ..................................................................... 19,0 |
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15. |
Podłoże Nogrady |
Pepton .................................................................. 10,0 Ekstrakt drożdżowy................................................ 6,0 NaCI ...................................................................... 5,0 Fosforan dwusodowy ........................................... 12,0 Fosforan jednopotasowy ........................................ 2,7 Dezoksycholan sodu .............................................. 4,0 Laktoza ............................................................... 12,5 Agar ..................................................................... 20,0 Błękit bromotymolowy .......................................... 0,1 Czerwień obojętna ................................................ 0,03 Mocznik ................................................................. 2,5 |
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14. |
Podłoże Mueller Hinton |
Hydrolizat kazeiny .............................................. 17,5 Ekstrakt wołowy ................................................... 2,0 Skrobia .................................................................. 1,5 Agar ..................................................................... 17,0 |
|
15. |
Agar na skosy |
Ekstrakt wołowy ................................................... 3,0 Ekstrakt drożdżowy............................................... 2,5 Aminobak .............................................................. 5,0 NaCI ...................................................................... 5,0 Glukoza ................................................................. 1,0 Agar ..................................................................... 15,0 |
|
16. |
Płyn do rozcieńczeń zbuforowany |
Pepton ................................................................... 1,0 NaCI ...................................................................... 8,5 NaHPO4 ............................................................ 0,044 NaH2PO4 ........................................................... 0,023 |
|
17. |
Podłoże Chapmana |
Wyciąg wołowy ..................................................... 1,0 Chlorek sodowy ................................................... 75,0 Polipepton ............................................................ 10,0 D-mannitol ........................................................... 10,0 Czerwień fenolowa ............................................ 0,025 Agar ..................................................................... 15,0 |
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18. |
Podłoże Perfringens base |
Pepton tryptonowy .............................................. 15,0 Pepton sojowy ........................................................ 5,0 Ekstrakt drożdżowy................................................ 5,0 Sodium metabisulphite .......................................... 1,0 Cytrynian żelazowo-amonowy III ......................... 1,0 Agar ..................................................................... 19,0 |
|
19. |
Podłoże Baird Parkera |
Pepton z kazeiny .................................................. 10,0 Ekstrakt mięsny . ................................................... 5,0 Ekstrakt drożdżowy ................................................1,0 Pirogronian sodowy ............................................. 10,0 Glicyna ................................................................. 12,0 Chlorek litu ............................................................ 5,0 Agar ..................................................................... .15,0 |
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20. |
Podłoże agarowe z fioletem, czerwienią, żółcią, glukozą
(podłoże VRBL) |
Ekstrakt drożdżowy ............................................... 3,0 Pepton .................................................................... 7,0 Chlorek sodu ......................................................... 5,0 Żółć wołowa .......................................................... 1,5 Laktoza ............................................................... 10,0 Czewień obojętna .................................................0,03 Fiolet krystaliczny .............................................. 0,002 Agar ..................................................................... .12,0 |
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21. |
Podłoże Eijkmana |
Aminobak ............................................................ 10,0 Laktoza ................................................................ 5,0 NaCI ..................................................................... 5,0 Purpura bromokrezolowa ..................................... 0,01
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|
22. |
Podłoże Sołtysa |
Ekstrakt drożdżowy ............................................. 1,25 Bulion ................................................................... 15,0 Taurocholan sodowy ............................................ 2,50 Zieleń brylantowa .................................................. 0,7 Fuksyna kwaśna .................................................... 0,5 Czerwień obojętna ............................................... 0,05 Agar ...................................................................... 10,0
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23. |
Woda tryptonowa
|
Pepton tryptonowy ..............................................10,0 Chlorek sodu ........................................................ 5,0 Woda destylowana ..........................................1000 ml |
|
25. |
Bulion odżywczy |
Ekstrakt mięsny .................................................... 3,0 Pepton ................................................................. 10,0 Chlorek sodu ......................................................... 5,0 Glukoza ............................................................... 10,0 Woda destylowana ..........................................1000 ml |
|
26. |
Podłoże YGC |
Ekstrakt drożdżowy ............................................... 5,0 Glukoza .................................................................20,0 Chloranfeniokol ..................................................... 0,1 Agar ......................................................................14,5 Woda destylowana ..........................................1000 ml |
|
27. |
Pożywka Voges-Proskauera |
Pepton .................................................................... 7,0 Glukoza ...................................................................5,0 Wodofosforan dwupotasowy .................................. 5,0 Woda destylowana ..........................................1000 ml |
|
28. |
Pożywka do wykrywania dekarboksylacji lizany
(bulion z lizyną) |
Chlorowodorek L-lizyny ....................................... 5,0 Ekstrakt drożdżowy ............................................... 3,0 Glukoza .................................................................. 1,0 Purpura bromokrezolowa ................................... 0,015 Woda destylowana ..........................................1000 ml |
|
29. |
Podłoże Muller-Kauffmana-Novobiocin |
Ekstrakt mięsny ..................................................... 4,3 Novobiocyna ......................................................... 0,04 Enzym. hydrol. kazeiny ......................................... 8,6 Chorek sodu ........................................................... 2,6 Zieleń brylantowa ..................................,.......... 0,0096 Węglan wapnia .................................................... 38,7 Tiosiarczan sodu bezwodny ................................ 30,5 Żółć wołowa suszona ............................................ 4,78 Woda destylowana ..........................................1000 ml |
|
30. |
Bulion Rappaport-Vassiliadis |
Enzymatyczny hydrolizat sojowy ………………. 5,0 Chlorek sodu ......................................................... 8,0 Diwodorofosforan potasowy ................................ 1,4 Wodorofosforan dipotasu ..................................... 0,2 Chlorek magnezowy ........................................... 40,0 Zieleń malachitowa ............................................... 0,04 Woda destylowana ..........................................1000 ml |
|
31. |
Pożywka Christensena |
Pepton .................................................................... 1,0 Glukoza .................................................................. 1,0 Chlorek sodowy ..................................................... 5,0 Ortofosforan jednopotasowy ................................. 2,0 Czerwień fenolowa ............................................ 0,012 Agar .............................................................. 12,0-18,0 Woda destylowana ..........................................1000 ml |
|
32. |
Agar trójcukrowy
(TSI) |
Pepton tryptonowy .............................................. 15,0 Pepton ................................................................... 5,0 Wyciąg mięsny ..................................................... 3,0 Wyciąg drożdżowy ............................................... 3,0 Chlorek sodu 5,0 ................................................... 5,0 Laktoza ................................................................ 10,0 Sacharoza ............................................................. 10,0 D +glukoza ............................................................ 1,0 Cytrynian amonowo-żelazowy /III/ ...................... 0,5 Tiosiarczyn sodu .................................................... 0,5 Czerwień fenolowa .............................................0,024 Agar ...................................................................... 12,0 Woda destylowana ..........................................1000 ml |
|
33. |
Pożywka z tioglikolanem sodu |
Trypton ............................................................... 15,0 L-cysteina ............................................................. 0,5 Glukoza ................................................................ 5,5 Ekstrakt drożdżowy .............................................. 5,0 Chlorek sodu ......................................................... 2,5 Tioglikoloan sodu ................................................. 0,5 Resazurina ..........................................................0,001 Agar ...................................................................... 0,75 Woda destylowana ..........................................1000 ml |
|
34. |
Podłoże Pseudomonas |
Pepton żelatynowy ............................................... 16,0 Hydrolizat kazeiny ............................................... 10,0 Siarczan potasu ................................................... 10,0 Chlorek magnezu ................................................... 1,4 Agar ..............................................................12,0-18,0 Woda destylowana ..........................................1000 ml |
|
35. |
Dodatek do podłoża Pseudomonas |
Cetrymit ............................................................... 5 mg Fucidyna ............................................................. 5 mg Cefaloridyna ......................................................25 mg |
|
36. |
Bulion sojowy z extraktem drożdżowym dla Listeria TSYEB |
Pepton tryptonowy .............................................. 17,0 Pepton sojowy ....... ............................................... 3,0 Glukoza ................................................................. 2,5 Chlorek sodu .......................................................... 5,0 Wodoroortofosforan dwupotasu ............................. 2,5 Ekstrakt drożdżowy ............. ...................................6,0 Woda destylowana ..........................................1000 ml |
|
37. |
Podłożę tryptono-żółciowe glukuronidynowe TBX |
Enzymatyczny hydrolizat kazeiny ........................ 20,0 Sole żółciowe nr3 ……….....……..………..….... 1,5 Kwas 5-bromo-4chloro-3-indolilo B-D-glukuronowy ......................................................................144 umol Sulfotlenek diemetylu .......................................... 3 ml Agar .......................................................... 9,0 do 18,0 Woda ............................................................ 1000 ml |
|
38. |
Bulion z azydkiem sodowym i fioletem krystalicznym |
Pepton tryptonowy .............................................. 20,0 Chlorek sodu .......................................................... 5,0 Wodorofosforan dwupotasowy .............................. 2,7 Dwuwodorofosforan potasu ................................... 2,7 Glukoza ................................................................ 15,0 Azydek sodu .......................................................... 0,4 Fiolet krystaliczny ........................................ 0,0012,5 Purpura bromokrezolowa . ................................/0,032 Woda destylowana ..........................................1000 ml |
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39. |
Agar wg. Mossela MYP |
Ekstrakt wołowy .................................................. 1,0 Pepton ................................................................... 10,0 D-mannitol ............................................................ 10,0 Chlorek sodu ........................................................ 10,0 Czerwień fenolowa ............................................ 0,025 Agar ..................................................................... 12,0 Woda destylowana ..........................................1000 ml |
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Roztwór polimyxyny B |
Siarczan B ......................................................... 1 mg Woda destylowana .............................................1 ml |
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Emulsja żółtka jaja kurzego ............................. 10 ml |
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40. |
Agar BHI |
Ekstrakt mózgowy, ekstrakt z serca i heptozy ...... 27,5 D(+) glukoza ......................................................... 2,0 Chlorek sodowy ..................................................... 5,0 Wodoroortofosforan disodowy .............................. 2,5 Agar -agar ........................................................... 15,0
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Suplement |
Chlorowodorek tiaminy |
|
41. |
Agar BHJ |
Wzbogacony chlorowodorkiem tiaminy 0,2 mg/l |
|
42. |
Podłoże Wrzoska
+ supplement |
Pepton........................................................................................10,0 Chlorek sodu .............................................................................5,0 Glukoza .....................................................................................5,0 Wyciąg z wątroby .....................................................................4,0
Suszone liofilizowane kostki wątroby woł. |
|
43. |
Test agar ph 6 |
Pepton kazeinowy trawiony trypsyną ...................................3,45 Pepton mięsny trawiny trypsyną ...........................................3,45 Chlorek sodu ...........................................................................5,1 Agar .........................................................................................13,0 |
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44. |
Test agar o ph 8 |
Pepton kazeinowy trawiony trypsyną .....................................3,45 Pepton mięsny trawiony trypsyną ...........................................3,45 Chlorek sodu ............................................................................5,1 12-hydratortofosforan trisodowy ..............................................2,4 Agar .........................................................................................13,0 |
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45. |
Bulion z siarczanem sodowo-lauryl. |
Pepton trypto..........................................................................20,0 Laktoza ...................................................................................5,0 Ortofosforan dwupotasowy ...................................................2,75 Ortofosforan jednopotasowy .................................................2,75 Chlorek sodu ..........................................................................5,0 Siarczan sodowo-laurylowy ...................................................0,1 |
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46. |
Podłoże VRBG |
Pepton ....................................................................................7,0 Extrakt drożdżowy ..................................................................3,0 Sole żółci 3 .............................................................................1,5 Glukoza ................................................................................10,0 Chlorek sodu ...........................................................................5,0 Czerwień obojętna .................................................................0,03 Fiolet krystaliczny .................................................................0,002 Agar .................................................................................9,0-18,0 |
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47. |
Podłoże do oznacz. ogól. liczby drobnoust. |
Pepton kazeinowy....................................................................5,0 Extrakt drożdżowy ..................................................................2,5 Glukoza ...................................................................................1,0 Agar ......................................................................................14,0 |
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48. |
Podłoże do oznacz. ogól. liczby drobnoustrojów w mleku |
Pepton kazeinowy ....................................................................5,0 Extrakt drożdżowy ..................................................................2,5 Glukoza ...................................................................................1,0 Mleko chude wolne od antybiotyków .....................................1,0 Agar .....................................................................................14,0 |
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49. |
Podłoże z żółcią, glukozą, zielenią brylantową EE bulion |
Pepton ......................................................................................10,0 Glukoza ....................................................................................5,0 Wodorofosforan disodu (Na2HPO4) ......................................6,45 Dwuwodorofosforan potasu ( KH2PO4)....................................2,0 Żółć wołowa ............................................................................20,0 Zieleń brylantowa ...............................................................0,0135 |
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50. |
Podłoże EC |
Pepton kazeinowy ................................................................20,0 Sole żółci ................................................................................1,9 Fosforan jednopotasowy ........................................................1,5 Laktoza ...................................................................................5,0 Fosforan dwupotasowy ..........................................................4,0 |
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51. |
Agar Slanetz-Bartley Z TTC |
Pepton kazeinowy ..................................................................15,0 Pepton sojowy .........................................................................5,0 Extrakt drożdżowy ..................................................................5,0 D-glukoza .................................................................................2,0 Fosforan potasu jednozasadowy .............................................4,0 Azydek sodu ............................................................................0,4 Chlorek 2,3,5- trifenylotetrazoliowy ......................................0,1 Agar ......................................................................................10,0 |
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52. |
Agar MRSV
supplement |
Tryptosa ...........................................................................4,59 Hydrolisat kazeinowy .........................................................4,59 Chlorek sodu ......................................................................7,34 Diwodorofosforan potasu ..................................................1,47 Chlorek magnezu odwodniony ........................................10,93 Zieleń malachitowa ............................................................0,037 Agar ....................................................................................2,7
Novobiocyna 20 mg |
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53. |
Płyn fizjologiczny z peptonem |
Pepton .................................................................................1,0 Chlorek sodu .......................................................................8,5
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54. |
Podłoże Endo |
Pepton ..................................................................................10,0 Laktoza .................................................................................10,0 Siarczan sodu ..........................................................................2,5 Fosforan dwupotasowy ...........................................................3,5 Fuksyna ..................................................................................0,4 Agar ........................................................................................10,0
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55. |
Pożywka ALOA Osttaviani- Agosti
supplementy |
Pepton ..................................................................................18,0 Trypton. ................................................................................6,0 Extrakt drożdżowy ..............................................................10,0 Pirogronian sodu....................................................................2,0 Glukosa ............................................................................... 2,0 Glicerofosforan magnezu.......................................................1,0 Siarczan magnezu bezwodny ................................................0,5 Chlorek sodu .........................................................................5,0 Chlorek litu ...........................................................................10,0 Wodorofosforan(V) disodu bezwodny .................................2,5 5-bromo-4-chloro -3-indolilo-b-D-glukopiranozyd..............0,05 Agar ..................................................................................12-18,0
r-r kwasu nalidyksowego.-0,02 w 5 ml wodorotlenku sodu 0,05 mol/l r-r ceftazidiminy- 0,02 w 5 ml wody siarczan polimyksyny B -76 700 IU w 5 ml wody r-r cykloheksemidyny -0,05 w 5 ml wody z alkohol etyl.1:1,lub r-r Amfoterycyny B 0,01 w 2,5 ml HCL(1 mol/l) i w7,5 ml DMF-dimetyloformamid kw. Mrówkowego r-r L-a-fosfstydiloinozytolu -2,0 w 50 ml wody |
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56. |
Podłoże Smith-Lorenza |
Pepton K ................................................................................10,0 Glukoza ..................................................................................5,0 Skrobia rozpuszczalna ..........................................................5,0 Purpura bromokrezolowa ......................................................0,04 Agar .....................................................................................15,0 |
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57. |
Agar MacConkey |
Pepton ................................................................................... 20,0 Laktoza ................................................................................. 10,0 Sole żółciowe ......................................................................... 1,5 Chlorek sodu .......................................................................... 5,0 Czerwień oboj. ....................................................................... 0,03 Fiolet krystaliczny ..................................................................0,001 Agar ..................................................................................... 15,0 |
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58. |
Bulion z Żółcią, laktozą i zielenią brylantową |
Pepton ................................................................................... 10 Laktoza ................................................................................. 10,0 Żółć wołowa sucha ............................................................... 20,0 Zieleń brylantowa ................................................................. 0,0133
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59. |
Pożywka agarowa tryptonowo-sojowa |
Trzustkowy hydrolizat kazeiny (tryptone) ........................... 15,0 Extrakt sojowy (sojtone) ....................................................... 5,0 Chlorek sodowy ..................................................................... 5,0 Agar ..................................................................................... 15,0 |
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