117828214

117828214



Acta Chromałographica 21(2009)4, 513-530 DOI: 10.1556/AChrom. 21.2009.4.1

Original Research Papers

Comparative Analysis of the Chromatographic Fingerprints of Twenty Different Sagę

(Salvia L.) Species

M. Daszykowski1, M. Sajewicz2, J. Rzepa2, M. Hajnos3, D. Staszek2,

Ł. Wojtal2, T. Kowalska2, M. Waksmundzka-Hajnos4, and

B. Walczak1 *

1 Department of Chemometrics, Institute of Chemistry, Silesian University,

9 Szkolna Street, 40-006 Katowice, Poland 2Department of General Chemistry and Chromatography, Silesian University,

9 Szkolna Street, 40-006 Katowice, Poland 3Department of Pharmacognosy, Medical University of Lublin,

1 Chodźki Street, 20-093 Lublin, Poland 4Department of Inorganic Chemistry, Medical University of Lublin,

6 Staszica Street, 20-081 Lublin, Poland E-mail: beata@us.edu.pl

Summary. Comparative analysis of twenty different sagę (Salvia L.) species grown in Poland has been performed on the basis of two types of chromatographic fingerprints. For efficient preprocessing and comparison of these fingerprints, chemometric methods were used. The main emphasis was on preprocessing of herbal fingerprints and selecting a suitable preprocessing strategy for exploring differences among them. After successful preprocessing of the fingerprints, principal component analysis was used to reveal Chemical differences among the samples. An outcome of the comparative analysis was to pinpoint specific regions of the fingerprints indicative of differences among the samples. In fingerprints of the volatile fraction from the sagę (Saluia L.) species, obtained from head-space gas chromatography coupled with mass spectrometry, important regions were identified and associated with the presence of camphene, limonene, and eucalyptol in these samples.

Key Worda: background removal, chemometrics, comparative analysis, denoising, sig-nal processing, sagę (Salvia L.) species, HPLC fingerprints, HS-GC-MS fingerprints

Introduction

The genus Salvia is recognized around the world for having commercial, medicinal and cultural importance, mainly because of the useful essential oils produced by Salvia foliage [1, 2]. Several Salina species have been culti-vated as ornamental plants, or for their aromatic and aromatherapeutic properties, for confectionery, and as culinary herbs [3]. About thirty Sahria species grow naturally or are cultivated in Poland [4). Medicinal applica-0231-2522 © 2009 Akademiai Kiadó, Budapest



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