117828249

Acta Chromatographica 23(2011)1, 181-190 DOI: 10.1556/A Chrom. 23.2011.1.13

Short Communication

Exploration of the Possibility of Combined HS-GC-MS and ¹³C NMR Spectroscopic Investigation of the Composition of the Essential Oils of Selected Sagę (Salvia) Species

M. Sajewicz¹, M. Matlengiewicz¹-², J. Rzepa¹, Ł. Wojtal¹, M. Hajnos³, M. Waksmundzka-Hajnos⁴, and T. Kowalska¹ *

Unstitute of Chemistry, University of Silesia 9 Szkolna Street, 40-006 Katowice, Poland ²Polish Acadcmy of Sciences, Centre of Poły mer and Carbon Materials,

5 Sowińskiego Street, 44-121 Gliwice, Poland ³Department of Pharmacognosy, Medical University of Lublin,

1 Chodźki Street, 20-093 Lublin, Poland ⁴Department of Inorganic Chemistry, Medical University of Lublin,

6 Staszica Street, 20-081 Lublin, Poland E-mail: [e-mail]

Summary. The objective of this study was to explore the possibility of combining head-space gas chromatography with mass spectrometric detection (HS-GC-MS) and ¹³C NMR spectroscopy to enhance the detectability of components of the essential oils of medicinal plants of the Saluia genus. Preliminary investigations were performed with two sagę species, Saluia laoandulifolia and Saluia triloba, known for particularly abundant yields of their respective essential oils. By use of HS-GC-MS, characteristic fingerprints of the volatile fractions were obtained for plant species from two different vegetation seasons (2007 and 2008). Partial identification of the components of these volatile fractions was performed, and comparison of the chromatographic fingerprints confirmed seasonal fingerprint similarity within the same species. Preliminary ¹³C NMR measure-ments resulted in well shaped spectra with an abundance of regularly distributed sig-nals. This suggested the possibility of using ^,3C NMR spectroscopy in phytochemical re-search, in parallel with HS-GC-MS, to enhance the detectability of volatile components of plant species belonging to this genus.

Key Words: essential oils, Salvia lawndulijblia, Sahia triloba, headspace-gas chromatog-raphy-massspectrometry, ^l3C NMR spectroscopy

Introduction

The heaith benefits of sagę (Salvia) essential oils can be attributed to a wide spectrum of curative properties of species belonging to this genus, in par-ticular their antifungal [1], antimicrobial [2], antioxidant, antiseptic, anti-irtflammatory, antispasmodic, antibacterial, cholagogue, choleretic, cicatri-

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