3870137286

ZASTOSOWANIE SPEKTROSKOPII JĄDROWEGO REZONANSU MAGNETYCZNEGO 5

ABSTRACT

The title problem is reviewed in the three main areas of generał interest to che-mists, biochemists and biologists, i.e. mutual interactions of smali molecules, ligand to biomolecule bindingand interactions ofbiomolecules. Physical phenomena, speci-fic and nonspecific, such as hydrogen bonding, self association, hydrophobic interactions, etc., are shown as being defined by NMR spectral parameters in solution and solid state.

Diversity of hydrogen bonding encountered in naturę is exemplified by the in-teraction of acetic acid with Watson-Crick vs Hoogsteen faces of adenosine, studied by DNMR in organie solvent at Iow temperatures. The complementarity of solid state NMR to solution studies of defining the naturę of hydrogen bonding is presen-ted in the case of genistein interaction with nitrogen bases.

Application of a title method is presented for searching the geometry of com-p!exes formed of organie ligands and biological molecules. General protocol for searching the geometry of complex is presented for the case of topotecan (TPT) interaction with duplex octamer and nicked decamer duplexes of DNA.

Interaction ofbiomolecules is presented for the case of duplex octamer DNMR study showing effects of base pairing and stacking on the S ^lH values and insulin aggregation as studied by Pulse Field Gradient NMR spectroscopy leading to rela-ting the radius of molecular aggregate with the diffusion constants.

In conclusion it is stated that various NMR techniques in solid and Iiquid state, coupled with X—ray and MS, constitute modern tool of structural biology in pursue of disclosing functions ofbiomolecules expressed by their interactions.

Keywords: intermolecular interactions, biomolecules, organie ligands, drugs, NMR

Słowa kluczowe: oddziaływania międzycząsteczkowe, biocząsteczki, ligandy organiczne, leki, NMR