Postępy Biochemii 58 (4) 2012
429
Anna Cmoch
1,*
Patrick Groves
2
Małgorzata Palczewska
2
Sławomir Pikuła
1
1
Department of Biochemistry, Nencki Insti-
tute of Experimental Biology, Polish Acad-
emy of Sciences, 02-093 Warsaw, Poland
2
Department of Biological Chemistry, Instituto
de Tecnologia Quimica e Biologica, Universi-
dade Nova de Lisboa, Av. da Republica, 2780-
157 Oeiras, Portugal
*
Department of Biochemistry, Nencki Institute
of Experimental Biology, Polish Academy of
Sciences, 3 Pasteura Street, 02-093 Warsaw,
Poland; e-mail: a.cmoch@nencki.gov.pl
Received: October 10, 2012
Accepted: October 29, 2012
Key words: S100, annexins, signal transduc-
tion, calcium ions, calcium binding proteins
Abbreviations: AnxA — vertebrate annexin;
[Ca
2+
] — Ca
2+
concentration; CaBPs — calcium
binding proteins; ER — endoplasmic reticulum
Acknowledgements: This work was sup-
ported by a grant N N401 140639 from the Pol-
ish Ministry of Science and Higher Education
and by Polish-Portugal Executive Program for
years 2011-2012 (project 760) sponsored by the
Polish Ministry of Science and Higher Educa-
tion and by Portuguese Fundação para a Ciên-
cia e a Tecnologia.
S100A proteins in propagation of a calcium signal in norm and pathology
ABSTRACT
C
alcium ions are essential factors controlling the balance between cell survival, growth,
differentiation and metabolism. Ca
2+
acts as a global second messenger involved in the
regulation of all aspects of cell function. Fluctuations in the intra- and extracellular Ca
2+
con-
centration [Ca
2+
] in response to different environmental stimuli drive most cellular func-
tions. Therefore, sustenance of calcium homeostasis requires perfect organization in time
and space that is achieved by calcium binding proteins (CaBPs). These proteins are involved
in sensing and transforming calcium signals to downstream cellular responses. Growing
number of evidence suggests than many human disorders, including cancer progression, are
related to deregulation of cellular calcium homeostasis and defects in CaBPs functions. In
this review we will focus on the roles of S100A proteins in intracellular and extracellular
calcium signalling and homeostasis. The S100A subfamily is among the most distinctive of
EF-hand CaBPs and are found exclusively in vertebrates. They are believed to have evolved
to enable activation of specific biochemical pathways in parallel to the activity of Ca
2+
sen-
sors such as calmodulin and/or annexins. The importance of S100 proteins is underscored by
their deregulated expression in neurodegenerative and inflammatory disorders, myopathies
and cancer. In addition, S100 proteins serve as diagnostic markers in the clinic and are under
constant investigation. Their roles and the roles of the S100A protein partners in normal and
pathology will be also discussed.
InTroduCTIon
The role of Ca
2+
as a key and pivotal second messenger in cells depends largely
on a wide number of heterogeneous calcium binding proteins. The S100 family
of proteins comprises 25, homologous, acidic calcium binding proteins with the
EF-hand type (helix-loop-helix motif) calcium binding domain. The canonical
C-terminal calcium-binding EF- hand is common to all EF-hand proteins, whi-
le the N-terminal EF-hand is non-canonical. The N-terminal EF-hand exhibits a
different architecture with a specific 14 amino acid motif flanked by helices HI
and HII. This motif is characteristic for S100 proteins and therefore it is often cal-
led ‘S100-specific’ or ‘pseudo EF-hand’. S100 proteins are characterized by low
molecular weights (9–13 kDa as monomers). They possess broadly known abi-
lity to form oligomers (homodimers, heterodimers and oligomeric assemblies)
and are characterized by tissue and cell-specific expression [1-4]. There is a great
diversification of the identified S100 proteins, but solely they are present only in
vertebrates. S100 genes were not found in such model organism as Arabidopsis
thaliana, Drosophila melanogaster, Caenorhabditis elegans or Saccharomyces cerevisiae.
In evolutionary terms, the lowest organism reported thus far containing a pseu-
do EF- hand protein closely related to S100A is a chondrichthye (dogfish Squalus
acanthias) [3].
The adopted nomenclature designates the S100 genes in the chro-
mosomal cluster 1q 21 as S100A followed by Arabic numbers (S100A1, S100A2
etc.). Several S100 proteins are present in human, but absent in rat and mouse
(S100A2, S100A12). There is also gene duplication (human S100A7) supporting
the hypothesis of the rapid evolution and expansion of the S100 family of pro-
teins [5].
S100A proteins possess ability to form higher complexes (homodimers, hete-
rodimers and oligomeric assemblies) and are characterized by tissue and cell-
-specific expression [3,4]. Up to date, several heterodimeric S100 proteins have
been reported: S100B forms heterodimers with S100A1, S100A6 and S100A11;
S100A1 with S100A4, S100P and S100A7 with S100A10. Noncovalent multimers
were observed for S100A12, S100A8 ⁄A9, S100A4 and a Zn
2+
-dependent tetramer
for S100A2 [3].
The conformation, folding and oligomerization state of S100s are responsive
to their metal-binding properties and have a pivotal influence on their function.
S100 proteins exert their actions usually through Ca
2+
binding, although Zn
2+
and Cu
2+
have also been shown to regulate their biological activity. Binding of
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Ca
2+
to S100s exposes hydrophobic sites, which enable them
to interact with specific target proteins or membranes. Bin-
ding of the target protein in the presence of calcium often
results in an increase in calcium affinity of the S100 prote-
in as well [6-8]. Most S100 proteins are directly involved in
intracellular calcium signal transduction, Ca buffering and
in Ca uptake and transport. At low cytosolic [Ca
2+
], as in
the resting state of the cell, S100 proteins possess a closed,
relatively hydrophilic conformation. During cell activation
the cytosolic [Ca
2+
] increases due to Ca
2+
influx via plasma
membrane Ca
2+
channels and exchangers or due to their
release from intracellular Ca
2+
stores such as endoplasmic
reticulum (ER) and mitochondria. S100 proteins are charac-
terized by affinites for Ca
2+
, e.g. in a range that allows them
to respond to changes of cytosolic [Ca
2+
] (with one exception
of S100A10, which is Ca
2+
insensitive) [9-11]. The resulting,
Ca
2+
-dependent structural changes largely affect helix III
[12,13].
Intracellularly, S100 proteins act as Ca
2+
sensors, transla-
ting increases of cytosolic Ca
2+
level into a cellular respon-
se. S100 proteins display a relatively large range of calcium
binding affinity (K
D
20–500 µM). The binding of S100 pro-
teins to their targets is typically calcium-dependent, but
calcium-independent interactions have also been described
(S100A10-AnxA2). Evidence exists that Ca
2+
binding dicta-
tes the membrane binding affinity of S100A. Interestingly,
in some cases the interaction with membranes is weaker for
Ca
2+
bound S100A13 than in apo-S100A13 [14].
S100A2, S100A3, S100A6, S100A7, S100A8⁄9 and S100A12
bind Zn
2+
in specific structural sites. The binding of diffe-
rent metal ions results in conformational adjustments and
modulation of S100 protein folding and function in cells.
In the case of S100A12, Zn
2+
binding leads to an increase in
Ca
2+
affinity, whereas in S100A2 the opposite
effect was observed. On the other hand, Zn
2+
and Ca
2+
binding to some of S100 proteins
are both required for their interaction with
receptors such as RAGE (the receptor for ad-
vanced glycation end-products). The S100A5,
S100A12 and S100A13 binds Cu
2+
at the same
sites to which Zn
2+
binds [3].
InTrA- And EXTrACELLuLAr
PArTnErS oF S100A ProTEInS
Members of the S100 family of proteins, in
the calcium dependent or independent man-
ner, interact with a variety of target proteins
including enzymes, cytoskeletal subunits,
receptors, transcription factors and nucleic
acids. Several S100 proteins exhibit Ca
2+
-de-
pendent interactions with metabolic enzymes
(S100A1 with aldolase C), with cytoskeletal
proteins (S100A1 with tubulin or with DNA-
-binding proteins, S100A2 and S100A4 inte-
ract with p53) [15].
S100 proteins are known to interact with
members of the other large family of cal-
cium binding proteins - annexins -(AnxA2
with S100A4, S100A6, S100A10 or S100A11
and AnxA6 with S100B, S100A6, S100A11,
S100A1) to form complexes that exhibit biological activities
[16-18] (Fig. 1).
S100A10 interacts not only with AnxA2 but also with
multiple proteins: serotonin receptor 5-HT1B, NaV1.8 so-
dium channel, TASK-1 potassium channel, ASIC-1 chan-
nels; TRPV5/TRPV6 channels, cytosolic phospholipase A
2
,
BAD (Bcl2-antagonist of cell death), AHNAK (neuroblast
differentiation-associated protein), cathepsin B, plasmi-
nogen activator, transglutaminase, S100A7, S100A8 [19].
Many functional consequences of the interactions between
S100A10 and its partners have been reported. There is accu-
mulating evidence that S100A10 interacts with a diverse set
of target proteins and regulates various biological functions
in different cellular compartments.
Most researchers concentrate on S100A10-annexin A2
heterotetramer formation and functions inside and outside
of the cell. Typically, S100A10 is found in most cells bound
to its annexin A2 ligand as the heterotetrameric S100A-
10
2
AnxA2
2
complex, AIIt. In addition to an intracellular di-
stribution, S100A10 is present on the extracellular surface
of many cells. It was indicated that it facilitates the translo-
cation of TRPV5 and TRPV6 channels towards the plasma
membrane in endothelial cells [20]. TRP (transient recep-
tor potential) channels constitute a superfamily of sensory
channels whose functions range from phototransduction
(where they were first described), olfaction, heat, cold sen-
sation etc., to Ca
2+
sensors/transporters. The TRP Ca
2+
chan-
nels are important for absorption of Ca
2+
into kidney, bone,
placenta, or intestine to maintain systemic Ca
2+
homeostasis.
The S100A10-annexin 2 complex specifically associates with
the C-terminal of TRP channels and is suggested to play a
Figure 1. STRING 9.0 analysis of direct (physical) or indirect (functional) associations between human
annexins and S100s proteins. The lines represent the existence of the several types of evidence used in
predicting the associations (high confidence score 0.7). The interactions are shown in different colors:
black is co-expression, dark blue is co-occurrence, purple is experimental evidence, light green is text
mining.
Postępy Biochemii 58 (4) 2012
431
role in guiding and localizing channels to the plasma mem-
brane and/or in the modulation of channel activity. It ap-
pears to act as a scaffolding protein that conjugates appro-
priate proteins at the plasma membrane. S100A10-annexin 2
also interacts with acid-sensing ion channels (ASIC1a) [21].
S100A10 has been reported to modulate the activity of
NaV1.8 (tetrodotoxin-resistant voltage-gated sodium chan-
nel), which is involved in the transmission of nociceptive
information from sensory neurons to the central nervous
system in nociceptive and neuropathic pain conditions.
NaV1.8 requires S100A10 accessory proteins for its functio-
nal expression on the plasma membrane [22].
The AnxA2-S100A10 complex formation in some types of
cells leads to plasminogen activation, either tissue-type pla-
sminogen activator (tPA) or urokinase-type plasminogen
activator (uPA), facilitating the conversion of plasminogen
to plasmin. The formation of a ternary complex between
tPA, plasminogen, and S100A10 provides a mechanism to
localize the proteolytic activity of plasmin to the cell sur-
face. Active plasmin both degrades fibrin directly and ac-
tivates members of the matrix metalloproteases family,
creating a localized proteolytic hub during angiogenesis or
tumor growth [23,24]. Annexin A2 plays an important role
in plasminogen regulation by controlling the levels of extra-
cellular S100A10 and by acting as a plasmin reductase. The
mechanism by which annexin A2 regulates the extracellular
levels of S100A10 is unknown.
Some ion channels that are regulated by calmodulin
may in fact be modulated by S100 proteins, either under
resting conditions or under special circumstances. Whether
the effect of S100 proteins is direct or indirect, knowledge
of the molecular basis for calmodulin interactions with ion
channels may be helpful in discerning how S100 proteins
modulate ion channels, in particular since there may well
be a similarity in their mode of action [25]. Some K
+
, Na
+
,
and Cl
−
channels are activated or modulated by intracellular
Ca
2+
signals giving rise to the notion that Ca
2+
binding pro-
teins may play a role in regulating channel gating function.
There is growing evidence for modulatory roles played by
the S100 proteins in the regulation of those types of chan-
nels. External binding of S100 proteins to ion channels has,
however, not been reported so far [26].
S100A4, the recently recognized novel binding partner of
AnxA2, manages various functions dependent on cellular
compartmentalization. Intracellular S100A4 exists as a sym-
metric homodimer that facilitates the binding of its target
proteins (actin, nonmuscle myosin IIA and IIB, tropomy-
osin). Extracellular S100A4 interacts with AnxA2, MMP-13,
RAGE or epidermal growth factor (EGF) receptor ligands.
Through these interactions, S100A4 regulates cell mobility,
invasion, and angiogenesis [27,28]. S100A4 was reported
as involved in the regulation of osteoblastic transcription
factors Runx2/Cbfa1 and Osx. S100A4 plays an important
role in matrix remodeling by up-regulating the expression
of crucial matrix metalloproteinases (MMPs) of bones [29].
S100A1 is known to increase the L-type Ca
2+
channel (L-
-type Ca
2+
channels located in the SR) current at nanomolar
Ca
2+
concentrations and enhances Ca
2+
release in skeletal
and in cardiac muscle. S100A1 was shown to directly in-
teract with PKA and this complex appears to affect Ca
2+
channels. At the molecular level, S100A1 was shown to
interact in a Ca
2+
-dependent manner with the cardiac iso-
forms of RYR2 (ryanodine receptor, isoform 2) (Fig. 2) [30],
SERCA2A (sarco/endoplasmic reticulum calcium ATPase,
isoform 2A) [31], phospholamban (PLN), titin, and the mi-
tochondrial F1-ATP synthase in complex V of the respira-
tory chain [5].
S100A proteins have been also intensively studied for
their interaction with heat shock-regulated proteins. For
example, S100A6 mediates nuclear translocation of Sgt1
and its interaction with Hsp-90 [32,33]. S100A1 and S100A2
proteins regulate the Hsp-90 interaction with target proteins
[34]. S100A1 is also known to be a component of the Hsp70/
Hsp90 multichaperone complex [35].
CELLuLAr FunCTIonS oF S100A ProTEInS
A richness of possible targets for S100 proteins is in
accordance with their multiple functions. Therefore, S100
proteins regulate a diverse array of cellular activities,
including the differentiation [36] and apoptosis [37,38],
motility, membrane–cytoskeleton interactions and cyto-
skeleton dynamics [39,40], cellular Ca
2+
homeostasis [41],
transduction of intracellular Ca
2+
signals, innate and ada-
ptive immunity [1,2,25,42] and are predicted to partici-
pate in mineralization [43]. Up to now, only S100A4 and
S100A8/A9 have been considered as factors involved in
mineralization control [44-46]. They have been also re-
ported as cellular protectors from oxidative cell damage,
regulators of protein phosphorylation [47], secretion and
transcriptional factors [1-3]. S100A8/A9 complex, was re-
ported as a Ca
2+
sensor which controls the interplay be-
Figure 2. Molecular mechanisms of S100 target protein interactions. In the presen-
ce of elevated Ca
2+
concentrations, apo-S100 undergoes a conformational change
and interacts with target proteins in a Ca
2+
-dependent pathway. The interaction
of S100A1 and RyR receptors is shown as a specific example. In contrast, S100A10
interacts with annexin A2 independently of Ca
2+
.
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www.postepybiochemii.pl
and vascular smooth muscle cells, neurons, astrocytes,
Schwann cells, epithelial cells, myoblasts and cardiomy-
ocytes [49]. Multimeric assemblies seem to be necessary for
the extracellular functions of S100 proteins and have been
reported for S100A12, S100A4, S100B and S100A8/A9 [3].
S100-associated cell signalling may be promiscuous. This
can be best exemplified through S100A8 ⁄A9, which pro-
motes RAGE-dependent cell survival as well as multiple
RAGE-independent cell death pathways.
This diversity in function of the S100 provides evidence
of very extensive evolutionary optimization of the fit be-
tween the EF-hand CaBPs fold and the calcium ion. S100
proteins form a phylogenetically young group among the
EF-hand proteins (present only in verterbrates). Future rese-
arch in the coming years will certainly contribute to clarify
some of these and other questions and will ultimately bring
us to a higher level of understanding the biology of tumour
and degeneration and enable to use our acquired knowled-
ge of S100 structure and functions in developing strategies
to modulate their activity for therapeutic purposes [51].
rEGuLATIon oF S100A FunCTIonS BY
PoSTTrAnSLATIonAL ModIFICATIonS
A number of posttranslational protein modifications have
been described. Posttranslational protein modifications may
result in physiochemical changes of the protein with respect
to mass, charge, structure and conformation. S100 proteins
may be modified by various post-translational modifica-
tions, including phosphorylation, methylation, acetylation,
and oxidation. These modifications may alter their ion-bin-
ding properties, interactions with target proteins, transloca-
tion within cell compartments, degradation, protein-protein
interactions and extracellular functions.
tween extracellular Ca
2+
entry and intraphagosomal ROS
production [48].
Within the cells, S100 proteins often translocate from
one compartment to another (nucleus, cytoplasm) in re-
sponse to changes in calcium concentration or concentra-
tion of extracellular S100 using different translocation pa-
thways. In addition to their intracellular functions, S100
proteins can also be secreted and may exert the role of
cytokines (e.g. S100A4, S100A8, S100A9) through the ac-
tivation of various cell surface receptors in an autocrine
and paracrine manner through various receptors RAGE
(Fig. 3), toll-like receptor 4 (TLR-4) (Fig. 4), G-protein-co-
upled receptors, scavenger receptors or heparan sulfate
proteoglycans and N-glycans
[25]. S100B, S100P, S100A4,
S100A6, S100A8/A9, S100A11 and S100A12 are known to
interact with RAGE [49], while S100A8/A9 also bind Toll-
-like receptors or TLRs [50]. Some S100 proteins, including
S100A4, S100A7, S100A8 ⁄A9, S100A11, S100A12, S100B
and others, are commonly secreted, exhibiting cytokine-
-like and chemotactic activity. When S100A7, S100A8,
S100A9, S100A12 or S100B are secreted in response to cell
damage or activation, they act as alarmins (cellular stress
signals), activating other immune and endothelial cells.
Frequently, S100 poroteins are secreted upon Ca
2+
signa-
ling via vesicle fusion with the cell membrane into the
extra cellular space, where they might acquire oligomeric
structures specialized for extracellular functions [5].
S100 proteins can also be released into the extracellular
space in response to stimuli, or during cell damage, and
they promote responses including neuronal survival and
extension (S100B), apoptosis (S100A4 and S100A6), inflam-
mation (S100B, S100A8/A9, S100A11 and S100A12), au-
toimmunity (S100A8/A9), chemotaxis (S100A8/A9) and
cell proliferation and survival (S100P, S100A7), effectively
functioning as paracrine and autocrine mediators. Thus,
extracellular S100 proteins exert regulatory activities on
monocytes/macrophages/microglia, neutrophils, lym-
phocytes, mast cells, articular chondrocytes, endothelial
Figure 3. Native states and oligomerization pathways in S100 proteins. A scheme
outlining possible routes of oligomerization pathways for S100A8 and S100A9
proteins. From Fritz et al. [3], modified.
Figure 4. Binding of S100A to receptors in human disorders. Activation of RAGE
by S100 proteins leads to cell survival and proliferation. S100A8/A9 complex ac-
tivates TLR-4 receptors to induce inflammation. S100A8/A9 and S100A12 interact
with scavenger receptor. From LeClerc et al. [4], modified
Postępy Biochemii 58 (4) 2012
433
S100 proteins can be modified post-translationally by
phosphorylation (S100A8/A9, S100A11), nitrosylation
(S100A1, S100B, S100A8), citrullination (S100A3) (34), car-
boxymethylation (S100A8/A9), glutathionylation, trans-
amidation (S100A11) or sumoylation. These modifications
often modulate the interaction with calcium or target pro-
teins. Additionally, the promoters of several S100 proteins
have been found hypo- or hyper-methylated, resulting in
epigenetic changes in protein expression [25] (Fig. 5).
Extracellular functions of several S100s may be regula-
ted by oxidative modifications. Many redox-based signa-
ling pathways are regulated by reversible modifications
such as intra- and interprotein disulfides, S-nitrosylation
and glutathionylation. Cysteine S-nitrosylation is a new fac-
tor responsible for increasing functional diversity of S100A
proteins and helps explain the role of S100A as a Ca
2+
si-
gnal transmitter sensitive to NOS/redox state within cells.
S100A1 has been recently reported in PC12 cells to be endo-
genously S-nitrosylated at site important for target binding
[52]. S100A8 and S100A9 are S-nitrosylated by NO donors
including GSNO, the physiological regulator of NO trans-
port and signaling. S100A8 is preferentially nitrosylated in
the S100A8/A9 complex. S-nitrosylation of S100A9 is cal-
cium-dependent, whereas S100A8 is not. In contrast to the
proposed, protective role of extracellular S100A8 and to
some extent, S100A9, these proteins regulate intracellular
NADPH oxidase activity and thus, ROS (reactive oxygen
species) generation. Thus, there is a paradigm on dual roles
of S100A8/A9 in redox balance. Oxidation may represent a
switch, whereby the modified proteins display their func-
tions [53].
Phosphorylation of Ser and Thr residues increased the af-
finity of S100A for the p53 TAD domains. Conversely, acety-
lation and phosphorylation of the C-terminus of p53 decre-
ased the affinity for S100A2 [15]. S100A9 protein has been
also reported as phosphorylation target for MAPK [54].
Phosphorylation of that protein leads to S100A8/A9 hete-
rotetramer translocation and NOX2 activation. Kouno et al.
[55] proposed phosphorylated S100A11 to be recognized by
its target protein, nucleolin and in such complex transloca-
ted to the nucleus.
Sumoylation of two lysines in S100A4 molecule results in
nuclear translocation of this protein and its action as trans-
cription factor bound to the promoter region of MMP-13
[29,53]. Up to date S100 proteins have not been described to
be glycosylated.
dErEGuLATIon oF S100A GEnES EXPrESSIon
Deregulated expression of genes encoding members of
the S100 family of calcium-binding proteins has been asso-
ciated with multiple tumor types. S100A2, S100A4, S100A6
and S100A10 genes alterations have been associated with
brain tumors. Furthmore, the methylation/demethylation
of these genes plays a role in the control of their tumor gene-
rating potency [56]. S100A1 is thought to modulate the Ca
2+
sensitivity of the sarcoplasmic reticulum (SR) Ca
2+
release
channels (ryanodine receptors or RyRs) and chronic absence
of S100A1 results in enhanced L-type Ca
2+
channel activity
combined with a blunted SR Ca
2+
release amplification in
cardiomyocytes [57]. The loss of S100A10 from the extracel-
lular surface of cancer cells results in a significant loss in
plasmin generation. In addition, S100A10 knock-down cells
demonstrate a dramatic loss in extracellular matrix degra-
dation and invasiveness as well as reduced metastasis [58].
The specific knockdown of S100A4 strongly suppressed cell
growth, migration and invasion activities in cancer cells,
therefore S100A4 may positively regulate tumor cell proli-
feration, invasion and metastasis associated with multiple
molecules [59].
S100A ProTEInS In HuMAn dISEASES
— CLInICAL SIGnIFICAnCE
Members of the S100 protein family have been shown to
posess pathophysiologic implications. In addition, members
of the S100 protein family are extensively tested as useful
biomarkers of certain diseases and potential targets of clini-
cal therapies [60]. There is growing evidence that expression
of S100 proteins is altered in pathologies. The S100 protein
levels are associated with a wide array of pathological con-
ditions like chronic inflammation [1,2,61-65], immunode-
fence [27], cardiomyopathies [30,66-68], atherosclerosis [69],
rheumatoid arthritis [70,71], cystic fibrosis [72] and cancer
Figure 5. Intracellular and extracellular roles of S100A proteins. Shown are the best known targets/activities putatively regulated by S100A proteins in a Ca
2+
-dependent
and Ca
2+
-independent manner. From Donato et al. [1], modified.
434
www.postepybiochemii.pl
[1,2,61,73-89]. S100 proteins are thought to be also associa-
ted with diabetes and its complications [80], neurodege-
neration and Alzheimer disease [90,91] and posttraumatic
stress disorder [92].One of the most intringuing directions
in research on S100A proteins is their involvement in neuro-
nal plasticity regulation and depression [19].
Functions of some S100A proteins in cancer progression
are opposite to others, for example down-regulation of
S100A6 and S100A10 in breast cancer, irrespective of patho-
logical stage, was observed whereas S100A7, S100A8 and
S100A9 were strongly up-regulated only in some type of
breast cancer [93]. There are many factors that can modu-
late S100A expression in biological systems. For example,
the S100A10 coding gene is regulated by various factors:
dexamethasone, TGF-α, EGF, NO donors, interferon-γ, vita-
min D, retinoic acid, NGF, electroconvulsive treatment [19].
Up regulation of S100A10 occurs also in response to AnxA2
upregulation. Down-regulation of AnxA2 is coordinated
with lack of S100A10 protein, but the specificity of mecha-
nism which is responsible for that phenomenon still rema-
ins unknown. Here we provided the examples of complica-
ted regulation of S100A proteins functions and expression.
ConCLudInG rEMArKS And FuTurE PErSPECTIVES
The thesis is emerging that S100 proteins are phyloge-
netically new proteins displaying the unusual property of
acting both within cells as Ca
2+
sensor proteins implicated
in Ca
2 +
signal transduction, and outside cells as ligands for
specific cell surface receptors on an increasingly larger num-
ber of cell types. Therefore the grand challenge ahead is de-
termining the role of particular S100A proteins in diseases
related to Ca
2+
homeostasis disorder such as neurological
diseases and artheriosclerosis.
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www.postepybiochemii.pl
udział białek S100A w przekazywaniu sygnału
wapniowego w normie i patologii
Anna Cmoch
1,*
, Patrick Groves
2
, Małgorzata Palczewska
2
, Sławomir Pikuła
1
1
Zakład Biochemii, Instytut Biologii Doświadczalnej PAN im. M. Nenckiego, Warszawa, Polska
2
Zakład Chemii Biologicznej, Instytut Technologii Chemicznej i Biologicznej, Oeiras, Portugalia
*
e-mail: a.cmoch@nencki.gov.pl
Słowa kluczowe: białka S100, aneksyny, przekazywanie sygnałów, jony wapnia, białka wiążące wapń
STrESZCZEnIE
Jony wapnia są niezbędne w utrzymaniu równowagi pomiędzy procesami wzrostu, przeżywalności, różnicowania i metabolizmu komórki.
Jony wapnia odgrywają rolę przekaźnika II rzędu w niemal wszystkich procesach komórkowych. Zmiany zewnątrz- i wewnątrzkomórkowe-
go stężenia jonów wapnia, w odpowiedzi na pobudzenie, wpływają na funkcje komórek. utrzymanie homeostazy wapnia wymaga właściwej
organizacji wewnątrzkomórkowego rozmieszczenia wapnia, w czym uczestniczą białka wiążące jony wapnia. Białka te przekształcają sygnał
wapniowy w odpowiedź komórkową. Coraz większa liczba obserwacji świadczy o tym, że zaburzona homeostaza wapnia i nieprawidło-
wa funkcja białek wiążących jony wapnia jest przyczyną wielu chorób człowieka, w tym rozwoju nowotworów. W niniejszym artykule
przeglądowymi, omówiono funkcje białek S100A w procesie przekazywania sygnału wapniowego. Białka z tej rodziny występują tylko w
organizmach kręgowców. uczestniczą w specyficznych procesach komórkowych, równolegle z kalmoduliną i aneksynami. Ich znaczenie
podkreślają obserwacje świadczące, że ich poziom ulega zmianom w chorobach neurodegeneracyjnych, stanach zapalnych, miopatiach i w
różnych typach nowotworów. Białka S100A są również postrzegane jako wskaźniki kliniczne różnych chorób, co jest nadal przedmiotem
intensywnych badań. W przedstawionym artykule omówiono również białka partnerskie białek z rodziny S100A.
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