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G mutants efficiently mhibit host protein synthesis.

Having showed that VSV głycoprotein mutants could rapidly inhibit cellular transcription upon infection, we next assessed if this would correlate with their ability to inhibit cellular translation. We first determined that all G mutants efficiently synthesized their viral proteins in a pattem similar to that of wild-type VSV (Figures 3A, B). We then analyzed cellular protein synthesis during infection and observed an inhibition by all G mutants when compared to wild-type VSV although these differences did not reach statistical significance (Figurę 3C). In remarkable contrast, the M mutant showed only transient G protein synthesis and an inability to shutoff host protein synthesis compared to G mutants (P < 0.05 compared to Gs and P < 0.01 compared to other G mutants)