Wyznaczanie stałych równania kinetycznego reakcji izomeryzacji D – glukozy do D fruktozy(1)


Overview

Glukoza
Fruktoza
Termodynamika


Sheet 1: Glukoza

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

600 20x 15 30.00 1.789 1.595 1.542 1.69 0.137
S1 1.625 St1 0.641

7.5 44.00 1.595 1.552 1.686 1.64 0.064
S2 1.691 St2 0.658

0 68.00 1.63

1.63 0.000
S3 1.969 St3 0.663

<0 107.00 1.539 1.625 1.63 1.60 0.051
S śr 1.658 St śr 0.654











SD 0.047 SD 0.012

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

800 40x 15 34.40 0.964 0.847 0.835 0.96 0.000
S1 0.857 St1 0.627

7.5 49.50 0.951 0.958 0.935 0.95 0.012
S2 0.843 St2 0.589

0 69.50

0.898 0.90 0.000
S3 1.023 St3 0.590

<0 113.50 0.548 0.675 0.607 0.61 0.064
S śr 1.023 St śr 0.602











SD 0.000 SD 0.022

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

1250 50x 30 35.90 1.275 1.969 2.004 1.99 0.025
S1 1.901 St1 0.530

20 58.60 1.901 1.646 1.503 1.77 0.180
S2
St2


10 91.00 1.796
1.583 1.58 0.000
S3 1.510 St3 0.542

0 273.00 1.959 1.95 1.979 1.96 0.015
S śr 1.901 St śr 0.536











SD 0.000 SD 0.008

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

50 2x 80 55.53 0.439 0.43 0.427 0.43 0.006
S1 0.658 St1 0.244

60 76.14 0.630 0.654 0.641 0.65 0.009
S2 0.679 St2 0.250

40 117.26 0.676 0.677 0.641 0.66 0.021
S3 0.686 St3 0.260

20 396.97 0.606 0.61 0.623 0.62 0.009
S śr 0.674 St śr 0.251











SD 0.015 SD 0.008

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

100 3x 80 55.62 1.767 1.774 1.700 1.75 0.041
S1 1.966 St1 0.544

60 73.50 1.908 1.856 1.890 1.90 0.013
S2 2.026 St2 0.524

40 120.49 1.899 1.882 1.917 1.89 0.012
S3 1.978 St3 0.542

20 439.98 1.824 1.807 1.816 1.81 0.006
S śr 1.990 St śr 0.537











SD 0.032 SD 0.011

Cs [mM] Rozc. RP t [s] A1 A2 A3 A śr SD
Substrat Absorb. Standard Absorb.

1500 50x 30 32.98 0.797 1.466 0.959 1.07 0.349
S1 1.406 St1 0.860

20 51.52 1.433
1.350 1.39 0.059
S2 1.402 St2 0.750

10 90.13 1.318 1.391 1.680 1.35 0.052
S3 1.339 St3 0.823

0 186.97 1.715 2.395 2.482 2.20 0.420
S śr 1.404 St śr 0.842











SD 0.003 SD 0.026
















Cg [mM] Crz,g [mM] Cp [mM] t [s] V [ml/s] τ [s] α [%] r [mM]/s] rśr [mM]/s] Cs [mM] Rozcień. 1/Cs 1/rśr


388.073 380.275 -7.798 30.00 1.667 3.510 -2.05% -2.222 0.793 600 20 0.002 1.260


376.261 4.014 44.00 1.136 5.148 1.06% 0.780



373.853 6.422 68.00 0.735 7.956 1.69% 0.807



366.514 13.761 107.00 0.467 12.519 3.62% 1.099



480.399 509.801 29.402 34.40 1.453 4.025 5.77% 7.31
800 40 0.001



472.425 37.375 49.50 1.010 5.792 7.33% 6.45



447.508 62.292 69.50 0.719 8.132 12.22% 7.66



303.987 205.814 113.50 0.441 13.280 40.37% 15.50



1389.809 1329.991 -59.818 35.90 1.393 4.200 -4.50% -14.241
1250 50 0.001



1240.788 89.202 58.60 0.853 6.856 6.71% 13.010



1107.509 222.481 91.00 0.549 10.647 16.73% 20.896



1373.134 -43.144 273.00 0.183 31.941 -3.24% -1.351



25.782 40.245 14.463 55.53 0.900 6.497 35.94% 2.226 0.111 50 2 0.020 9.016


38.644 1.601 76.14 0.657 8.908 3.98% 0.180



39.668 0.577 117.26 0.426 13.719 1.43% 0.042



36.794 3.452 396.97 0.126 46.445 8.58% 0.074



73.244 83.432 10.188 55.62 0.899 6.508 12.21% 1.566
100 3 0.010



79.616 3.815 73.50 0.680 8.600 4.57% 0.444



79.260 4.172 120.49 0.415 14.097 5.00% 0.296



75.948 7.484 439.98 0.114 51.478 8.97% 0.145



478.610 625.668 147.059 32.98 1.516 3.859 23.50% 38.111 0.924 1500 50 0.001 1.082


620.098 5.570 51.52 0.970 6.028 0.89% 0.924



603.610 22.059 90.13 0.555 10.545 3.53% 2.092



979.204 -353.535 186.97 0.267 21.875 -56.51% -16.161






























Vmax 1.451





Km 603.846













































Sheet 2: Fruktoza

Cs [mM] RP t [s] A1 A2 A3 A śr SD


Absorb.














50.00 15 30.29 0.023 0.022 0.028 0.024 0.003

St1 0.473














7.5 43.04 0.027 0.037 0.044 0.036 0.009

St2 0.546














0 65.88 0.051 0.058 0.057 0.055 0.004

St3 0.503














<0 107.47 0.089 0.085 0.091 0.088 0.003

St śr 0.507
























SD 0.037














Cs [mM] RP t [s] A1 A2 A3 A śr SD


Absorb.














100 80 66.00 0.015 0.018 0.015 0.016 0.002

St1 0.458














60 68.40 0.082 0.080 0.070 0.077 0.006

St2 0.460














40 126.00 0.137 0.142 0.150 0.143 0.007

St3 0.458














20 552.00 0.643 0.690
0.667 0.033

St śr 0.459
























SD 0.001














Cs [mM] RP t [s] A1 A2 A3




Absorb.














200 80 61.20 0.195 0.2 0.208 0.201 0.007

St1 0.472














60 72.60 0.201 0.198 0.195 0.198 0.003

St2 0.495














40 129.00 0.338 0.325 0.327 0.330 0.007

St3 0.463














20 439.20 1.033 1.461 1.070 1.052 0.026

St śr 0.477
























SD 0.017














Cs [mM] RP t [s] A1 A2 A3




Absorb.














400 80 50.38 0.163 0.164 0.165 0.164 0.001

St1 0.569














60 67.00 0.328 0.363 0.357 0.349 0.019

St2 0.538














40 108.00 0.668 0.672 0.687 0.676 0.010

St3 0.469














20 301.00 1.670 1.636 1.681 1.662 0.023

St śr 0.554
























SD 0.022














Cs [mM] RP t [s] A1 A2 A3




Absorb.














600 30 33.21 0.492 0.498 0.506 0.499 0.007

St1 2.455














20 51.47 1.278 1.342 1.347 1.322 0.038

St2 2.448














10 84.44
2.173 2.190 2.182 0.012

St3 2.398














0 174.99 2.509 2.546 2.546 2.534 0.021

St śr 2.434
























SD 0.031














Cs [mM] RP t [s] A1 A2 A3




Absorb.














800 15 30.42 0.486 0.545 0.540 0.524 0.033

St1 0.685














7.5 47.52 0.408 0.355 0.427 0.397 0.037

St2 0.652














0 71.28 0.443 0.575 0.498 0.505 0.066

St3 0.641














<0 113.14 0.521 0.633 0.703 0.619 0.092

St śr 0.659
























SD 0.023














Cs [mM] RP t [s] A1 A2 A3




Absorb.














1000 30 34.75 1.090 0.92 0.816 1.005 0.120

St1 1.084














20 51.38 1.339 1.212 1.257 1.235 0.032

St2 1.078














10 75.57 1.334 1.407 1.255 1.371 0.052

St3 1.072














0 180.71 1.929 2.371 2.070 2.000 0.100

St śr 1.078
























SD 0.006














Cs [mM] RP t [s] A1 A2 A3




Absorb.














1250 15 31.79 0.546 0.753 0.789 0.546 0.000

St1 0.654














7.5 48.88 0.676 0.704 0.697 0.692 0.015

St2 0.630














0 70.53 0.749 0.739 0.703 0.721 0.025

St3 0.685














<0 111.66 0.953 1.015 0.997 0.975 0.031

St śr 0.656
























SD 0.028














Cs [mM] RP t [s] A1 A2 A3




Absorb.














1500 15 31.73 0.202 0.24 0.285 0.221 0.027

St1 0.439














7.5 44.83 0.271 0.183 0.179 0.271 0.000

St2 0.434














0 67.06 0.241 0.289 0.250 0.289 0.000

St3 0.511














<0 109.59 0.437 0.505 0.401 0.448 0.053

St śr 0.437
























SD 0.004














Cs [mM] RP t [s] A1 A2 A3




Absorb.














1750 15 32.04 0.165 0.269 0.227 0.196 0.044

St1 0.502














7.5 46.04 0.217 0.243 0.228 0.229 0.013

St2 0.498














0 68.21 0.270 0.283 0.283 0.279 0.008

St3 0.495














<0 110.77 0.456 0.486 0.445 0.462 0.021

St śr 0.498
























SD 0.004





























Cp [mM] t [s] V [ml/s] τ [s] α [%] r [mM/s] rśr [mM] Cs [mM] 1/Cs 1/rśr

Vmax Km
0.360 30.29 1.651 3.54393 0.72% 0.102 0.104 50 0.020 9.588
R=0,984 1.354 627.926
0.532 43.04 1.162 5.03568 1.06% 0.106
R=0,9997 1.437 641.614
0.818 65.88 0.759 7.70796 1.64% 0.106



1.306 107.47 0.465 12.57399 2.61% 0.104



0.262 66.00 0.758 7.722 0.26% 0.034 0.158 100 0.010 6.317



1.265 68.40 0.731 8.0028 1.26% 0.158



2.338 126.00 0.397 14.742 2.34% 0.159



10.898 552.00 0.091 64.584 10.90% 0.169



3.163 61.20 0.817 7.1604 1.58% 0.442 0.355 200 0.005 2.814



3.115 72.60 0.689 8.4942 1.56% 0.367



5.192 129.00 0.388 15.093 2.60% 0.344



16.545 439.20 0.114 51.3864 8.27% 0.322



2.222 50.38 0.992 5.89446 0.56% 0.377 0.568 400 0.003 1.759



4.734 67.00 0.746 7.839 1.18% 0.604



9.155 108.00 0.463 12.636 2.29% 0.725



22.525 301.00 0.166 35.217 5.63% 0.640



1.537 33.21 1.506 3.88557 0.26% 0.396 0.679 600 0.002 1.474



4.075 51.47 0.971 6.02199 0.68% 0.677



6.723 84.44 0.592 9.87948 1.12% 0.680



7.808 174.99 0.286 20.47383 1.30% 0.381
















5.957 30.42 1.644 3.55914 0.74% 1.674 0.812 800 0.001 1.232





4.512 47.52 1.052 5.55984 0.56% 0.812




5.748 71.28 0.701 8.33976 0.72% 0.689




7.041 113.14 0.442 13.23738 0.88% 0.532




6.992 34.75 1.439 4.06575 0.70% 1.720 0.868 1000 0.001 1.152




8.589 51.38 0.973 6.01146 0.86% 1.429




9.535 75.57 0.662 8.84169 0.95% 1.078




13.911 180.71 0.277 21.14307 1.39% 0.658




6.239 31.79 1.573 3.71943 0.50% 1.677 0.926 1250 0.001 1.080




7.911 48.88 1.023 5.71896 0.63% 1.383




8.239 70.53 0.709 8.25201 0.66% 0.998




11.141 111.66 0.448 13.06422 0.89% 0.853




3.797 31.73 1.576 3.71241 0.25% 1.023 0.955 1500 0.001 1.047




4.656 44.83 1.115 5.24511 0.31% 0.888




4.966 67.06 0.746 7.84602 0.33% 0.633




7.692 109.59 0.456 12.82203 0.51% 0.600




2.950 32.04 1.561 3.74868 0.17% 0.787 0.623 1750 0.001 1.606




3.452 46.04 1.086 5.38668 0.20% 0.641




4.194 68.21 0.733 7.98057 0.24% 0.526
















6.958 110.77 0.451 12.96009 0.40% 0.537

















Sheet 3: Termodynamika

Próbka Cg,0 Cg* (G+E)
Próbka Cg,0 Cg* (G+E)
Próbka Cg,0 Cg* (G+E)
Próbka Cg,0 Cg* (G+E)
Próbka Cg,0 Cg* (G+E)



1 1.437 0.880
4 1.331 0.848
7 1.569 0.870
10 1.517 0.702
13 1.294 0.815



2 1.610 0.820
5 1.502 0.838
8 1.469 0.833
11 1.328 0.642
14 1.394 0.772



3 1.537 0.859
6 1.533 0.794
9 1.495 0.771
12 1.443 0.667
15 1.479 0.826



Średnia 1.528 0.853
Średnia 1.455 0.827
Średnia 1.511 0.825
Średnia 1.429 0.670
Średnia 1.389 0.804



SD 0.087 0.030
SD 0.109 0.029
SD 0.052 0.050
SD 0.095 0.030
SD 0.093 0.029


































































































Próbka Cf [mM] Cg [mM] K



K z wyliczonych parametrow
VmaxG*KmF/VmaxF*KmG











1,2,3 0.675 0.853 0.791



1.073













4,5,6 0.628 0.827 0.760


















7,8,9 0.686 0.825 0.832


















10,11,12 0.759 0.670 1.133


















13,14,15 0.585 0.804 0.728


















16,17,18





















Średnia

0.778


















SD

0.164
































































*wyrzucono punkty zaznaczone na czerwono








































































0.9725





















1.34125














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