3870137038

PODSTAWOWE TECHNIKI EKSPERYMENTALNE SPEKTROSKOPII NMR W FAZIE riFk'; FI    109

ABSTRACT

General overview of most important NMR techniąues and applications for organie and bioorganic molecules in liquids is given. The sensitivity of NMR experi-ments is discussed with regard to nuclear properties, B₀ field, applied method of excitation and detection, and finally concentration. In the next chapter the principles and consequences of quadrature detection are discussed. The most important techni-ques of quadrature detection in directly and indirectly detected domains are exem-plified. In the next point the principles, advantages and disadvantages of coherence selection techniques via phase cycling and Pulsed Field Gradients are indicated and compared with respect to yarious applications.

In the last part of present article the most important homo- (COSY, TOCSY, NOESY, and ROESY) and heteronuclear (HSQC, HMQC/HMBC) techniques used in NMR investigations in liquids are briefly reviewed. The characteristic features and exper?mental problems of these methods are discussed with respect to usual applications in chemistry and biochemistry.

Keywords: NMR spectroscopy, sensitivity, PFG, multidimensional acquisition

Słowa kluczowe: spektroskopia NMR, czułość metody, impulsowy gradient pola B_o (PFG), widma wielowymiarowe